Background: Dentin hypersensitivity is an increasingly common complaint, especially among young people. It manifests as painful symptoms in areas of exposed dentinal tubules and is related to the patients' lifestyle. Aim: The present work aimed to address a protocol with chemical desensitizing agents in treatment of Dentin Hypersensitivity. Case report: The treatment was performed in a 22-year-old female patient who presented as the main complaint a moderate to intense degree sensitivity in anterior and posterior teeth. In the 1st and 2nd week, a neural action desensitizer based on potassium nitrate (Ultra EZ™ - Ultradent Products Inc) was used, while in the 3rd and 4th week, a desensitizer with an obliterating action based on calcium phosphates (Nano P™ - FGM Dental Products) was chosen. In the 5th week, another chemical occlusal desensitizer agent was applied, the 5% sodium fluoride varnish (Enamelast™ - Ultradent Products Inc). Results: The patient was successfully treated by the associative technique through neural and blocker desensitizers obtaining effective pain control in a18-month follow-up. Conclusion: The associative technique of different chemical desensitizers has shown extremely satisfactory results, which can be confirmed in the present case report.